Gloria Muday, Professor of Biology and Director of the Center for Molecular Signalling
Charles M. Allen Professor of Biology
Director, Center for Molecular Signaling
B.S. Virginia Polytechnic Institute and State University
Ph.D. Purdue University
Postdoctoral Research, Sandoz Agro Inc.
2814 Wake Downtown
(and 41 Winston Hall)
(336) 758-5316
muday@wfu.edu
Current Research Projects
→ CLICK TO VISIT LAB WEBSITE ←
Areas of Interest
Hormone and redox signaling, flavonols, biochemistry, and molecular biology
Research
The emphasis of research in the Muday lab is the understanding of hormone and reactive oxygen species (ROS) signaling. We are using genetic and molecular biological approaches to study the role of the plant hormone, ethylene, in plant growth and development and how ethylene and auxin signaling pathways interact. We are using a systems biology approach to identify the receptor and transcriptional signaling networks that control ethylene-modulated root development in the model species of Arabidopsis thaliana, including stimulation of root hairs, and inhibition of primary root growth, lateral root initiation, and gravitropic response. We are also examining the hormonal regulation of synthesis of flavonols, plant specialized metabolites that act as antioxidants. We are studying both the biochemical machinery by which flavonols are synthesized and how flavonols modulate ROS homeostasis in multiple developmental processes in both Arabidopsis and tomato. Our interests are in flavonol-modulated ROS homeostasis in root hair and lateral root development, abscisic acid signaling in guard cells to control stomatal aperture, and heat stress-induced ROS that impairs pollen tube elongation and plant reproduction.
Video: Undergraduates Peer Teaching Genetics and Biotechnology in the Public Schools
Selected Publications
see also [Pubmed List of Papers]
(bold-type-face names are students and/or lab members)
Ali, MF and Muday, GK (2024) Reactive oxygen species are signaling molecules that modulate plant development. Invited Review. Plant, Cell, and Environment. doi/10.1111/pce.14837
Postiglione AE, Delange AM, Ali MF, Wang EY, Houben M, Hahn SL, Khoury MG, Roark CM, Davis M, Reid RW, Pease JB, Loraine AE, Muday GK. Flavonols improve thermotolerance in tomato pollen during germination and tube elongation by maintaining ROS homeostasis. bioRxiv [Preprint]. 2024 May 7:2023.12.23.573189. doi: 10.1101/2023.12.23.573189. PMID: 38187649; PMCID: PMC10769439.
Khoury, MG, Berenhaut, KS, Moore, KE, Allen, EE, Harkey, AF, Muhlemann, JK, Craven, CN, Xu, J, Jains, SS, John, DJJ, Norris, JL, and Muday, GK. (2023) Partitioned Local Depth analysis of time course transcriptomic data reveals elaborate community structure. In silico plants: doi.org/10.1093/insilicoplants/diad018
Daryanavard, H, Postiglione, AE, Mühlemann, JK, Muday, GK (2023) Flavonols modulate plant development, signaling, and stress responses. Current Opinion in Plant Biology; doi.org/10.1016/j.pbi.2023.102350
Postiglione, AE and Muday, GK (2022) Abscisic Acid drives stomatal closure through increases in hydrogen peroxide in mitochondria: Plant Physiology, doi.org/10.1093/plphys/kiac601
Martin RE, Postiglione AE, Muday GK (2022) Reactive oxygen species function as signaling molecules in controlling plant development and hormonal responses. Current Opinion in Plant Biology, 69, 102293.
Martin RE, Marzol E, Estevez JM, Muday GK. (2022) Ethylene signaling increases reactive oxygen species accumulation to drive root hair initiation in Arabidopsis. Development. 2022 Jul 1;149(13):dev200487.
Postiglione AE, Muday GK. (2022) Abscisic Acid Increases Hydrogen Peroxide in Mitochondria to Facilitate Stomatal Closure. Plant Physiology. 2022 Dec 27.
Postiglione AE, Muday GK. (2022) Abscisic Acid Drives Stomatal Closure through Increases in Hydrogen Peroxide in Distinct Subcellular Compartments Including Mitochondria. bioRxiv. 2022.
Postliglione AE, Muday GK. (2022) Abscisic Acid Increases Hydrogen Peroxide in Multiple Subcellular Compartments to Drive Stomatal Closure. bioRxiv. 2022 Jan 1.
Chapman JM, Muday GK (2021) Flavonols modulate lateral root emergence by scavenging reactive oxygen species in Arabidopsis thaliana. Journal of Biological Chemistry 296, 100222.
Postiglione AE, Muday GK (2021) The Role of ROS Homeostasis in ABA-Induced Guard Cell Signaling. Frontiers in Plant Science 11
Gibson CL, Isley JW, Falbel TG, Mattox CT, Lewis DR, Metcalf KE, Muday GK. (2020) A Conditional Mutation in SCD1 Reveals Linkage Between PIN Protein Trafficking, Auxin Transport, Gravitropism, and Lateral Root Initiation. Frontiers in plant science 11, 910.
EE Allen, J Farrell, AF Harkey, DJ John, G Muday, JL Norris, B Wu (2020) Time Series Adjustment Enhancement of Hierarchical Modeling of Arabidopsis Thaliana Gene Interactions. International Conference on Algorithms for Computational Biology, 143-154
Gayomba SR, Muday GK. (2020) Flavonols regulate root hair development by modulating accumulation of reactive oxygen species in the root epidermis. Development. 2020;147(8):dev185819. Published 2020 Apr 27. doi:10.1242/dev.185819
Hildreth SB, Foley EE, Muday GK, Helm RF, Winkel BSJ. The dynamic response of the Arabidopsis root metabolome to auxin and ethylene is not predicted by changes in the transcriptome. Sci Rep. 2020;10(1):679. Published 2020 Jan 20. doi:10.1038/s41598-019-57161-9
Harkey AF, Yoon GM, Seo DH, DeLong A, Muday GK. (2019) Light Modulates Ethylene Synthesis, Signaling, and Downstream Transcriptional Networks to Control Plant Development. Front Plant Sci. 2019 Sep 12;10:1094
Chrispeels HE, Chapman JM, Gibson CL, Muday GK. (2019) Peer Teaching Increases Knowledge and Changes Perceptions about Genetically Modified Crops in Non-Science Major Undergraduates. CBE Life Sci Educ. 2019 Jun;18(2):ar14. doi: 10.
Chapman JM, Muhlemann JK, Gayomba SR, Muday GK. (2019) RBOH-dependent ROS synthesis and ROS scavenging by plant specialized metabolites to modulate plant development and stress responses. Chem Res Toxicol. 2019 Feb 20.
Mühlemann, JK, Younts, TL, Muday, GK (2018) Flavonols control pollen tube growth and integrity by regulating ROS homeostasis during high-temperature stress. Proc Natl Acad Sci USA115: E11188-E11197
Muday, GK and Brown-Harding, H (2018) Nervous system-like signaling in plants. Science: 361: 1068-1069
Harkey AF, Watkins JM, Olex AL, DiNapoli KT, Lewis DR, Fetrow JS, Binder BM, Muday GK. (2018) Identification of Transcriptional and Receptor Networks That Control Root Responses to Ethylene. Plant Physiol. 2018 Mar;176(3):2095-2118. doi: 10.1104/pp.17.00907. Epub 2017 Dec 19.
Watkins, JM, Chapman, JM, and Muday, GK (2017) Abscisic acid-induced reactive oxygen species are modulated by flavonols to control stomata aperture. Plant Physiology. 175, 1807-1825.
Jenks MZ, Fairfield HE, Johnson EC, Morrison RF, Muday GK. (2017) Sex Steroid Hormones Regulate Leptin Transcript Accumulation and Protein Secretion in 3T3-L1 Cells. Sci Rep. 2017 Aug 15;7(1):8232. doi: 10.1038/s41598-017-07473-5.
Li YH, Zhang HN, Wu QS, Muday GK. (2017) Transcriptional sequencing and analysis of major genes involved in the adventitious root formation of mango cotyledon segments. Planta. 2017 Jun;245(6):1193-1213. doi: 10.1007/s00425-017-2677-9. Epub 2017 Mar 16.
Zhang C, Brown MQ, van de Ven W, Zhang ZM, Wu B, Young MC, Synek L, Borchardt D, Harrison R, Pan S, Luo N, Huang YM, Ghang YJ, Ung N, Li R, Isley J, Morikis D, Song J, Guo W, Hooley RJ, Chang CE, Yang Z, Zarsky V, Muday GK, Hicks GR, Raikhel NV. 2016. Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis. Proc Natl Acad Sci U S A. 2016 Jan 5;113(1):E41-50.
Gerttula S, Zinkgraf M, Muday GK, Lewis DR, Ibatullin FM, Brumer H, Hart F, Mansfield SD, Filkov V, Groover A. 2015. Transcriptional and Hormonal Regulation of Gravitropism of Woody Stems in Populus. Plant Cell. 2015 Oct;27(10):2800-13.
Maloney GS, DiNapoli KT, Muday GK. 2014. The anthocyanin reduced tomato mutant demonstrates the role of flavonols in tomato lateral root and root hair development. Plant Physiol. 2014 Jul 8. pii: pp.114.240507.
Watkins JM, Hechler PJ, Muday GK. 2014. Ethylene-induced flavonol accumulation in guard cells suppresses reactive oxygen species and moderates stomatal aperture. Plant Physiol. 2014 Apr;164(4):1707-17.
Patton KL, John DJ, Norris JL, Lewis DR, Muday GK. 2014. Hierarchical Probabilistic Interaction Modeling for Multiple Gene Expression Replicates. IEEE/ACM Trans Comput Biol Bioinform. 2014 Mar-Apr;11(2):336-46.
Chrispeels HE, Klosterman ML, Martin JB, Lundy SR, Watkins JM, Gibson CL, Muday GK. 2014. Undergraduates achieve learning gains in plant genetics through peer teaching of secondary students. CBE Life Sci Educ. 2014 Winter;13(4):641-52. doi: 10.1187/cbe.14-01-0007.
Lewis DR, Olex AL, Lundy SR, Turkett WH, Fetrow JS, Muday GK. 2013. A Kinetic Analysis of the Auxin Transcriptome Reveals Cell Wall Remodeling Proteins That Modulate Lateral Root Development in Arabidopsis. Plant Cell. 2013 Sep 17.
Sukumar P, Maloney GS, Muday GK. 2013. Localized induction of the ATP-binding cassette B19 auxin transporter enhances adventitious root formation in arabidopsis. Plant Physiol. 2013 Jul;162(3):1392-405.
Muday GK, Rahman A, Binder BM. 2012. Auxin and ethylene: Collaborators or Competitors? Trends in Plant Science, 17: 181-195.
Miller GD, Jenks MZ, Vendela M, Norris JL, Muday GK (2012) Influence of weight loss, body composition, and lifestyle behaviors on plasma adipokines: a randomized weight loss trial in older men and women with symptomatic knee osteoarthritis. J Obes. 2012;2012:708505. doi: 10.1155/2012/708505. Epub 2012 Dec 26.
Grunewald W, De Smet I, Lewis DR, Löfke C, Jansen L, Goeminne G, Vanden Bossche R, Karimi M, De Rybel B, Vanholme B, Teichmann T, Boerjan W, Van Montagu MC, Gheysen G, Muday GK, Friml J, Beeckman T. 2012. Transcription factor WRKY23 assists auxin distribution patterns during Arabidopsis root development through local control on flavonol biosynthesis. Proc Natl Acad Sci U S A. 2012 Jan 31;109(5):1554-9.
Fernandez-Marcos M, Sanz L., Lewis DR, Muday GK, Lorenzo O. 2011. Nitric oxide causes root apical meristem defects and growth inhibition while reducing PIN1-dependent acropetal auxin transport. PNAS 1108644108
Lewis DR, Negi S, Sukumar P, Muday GK. 2011. Ethylene inhibits lateral root development and enhances IAA transport by altered expression and localization of auxin transport proteins. Development 138: 3485-3495.
Lewis DR, Ramirez MV, Miller ND, Keith R, Helm R, Winkel BSJ, Muday GK. 2011. Auxin and ethylene induce distinct flavonol accumulation patterns through independent transcriptional networks: Plant Physiol. 156: 144-164
Negi S, Sukumar P, Liu X, Cohen JD, Muday GK. 2010. Genetic dissection of the role of ethylene in regulating auxin dependent lateral and adventitious root formation in tomato. Plant Journal 61: 3-15
Lewis DR and Muday GK. 2009. Measurement of auxin transport in Arabidopsis thaliana Nature Protocols: 4: 437-451.
Sukumar, P, Edwards, KS, Rahman, A, and DeLong, A, and Muday, GK. 2009. PINOID kinase regulates root gravitropism through modulation of PIN2-dependent basipetal auxin transport in Arabidopsis thaliana. Plant Physiol: 150: 722-735.
Negi S, Ivanchenko MG, Muday GK. 2008. Ethylene regulates lateral root formation and auxin transport in Arabidopsis thaliana. Plant J. 55: 175-187
Ivanchenko MG, Muday GK, Dubrovsky JG. 2008. Ethylene-auxin interactions regulate lateral root initiation and emergence in Arabidopsis thaliana. Plant J. 55: 335-347
Buer CS, Muday GK, Djordjevic MA. 2008. Implications of long-distance flavonoid movement in Arabidopsis thaliana. Plant Signaling and Behavior: 3: 415-417
Muday GK and Rahman A. 2007. Auxin transport and the integration of gravitropic growth. In Plant Tropisms: eds Gilroy, S and Masson, P, Blackwell Publishing pp 47-78.
Buer CS, Muday, GK, Djordjevic MA. 2007. Flavonoids are transported long distances in Arabidopsis. Plant Physiol. 145: 478-490.
Muday GK, Brady, SR, Argueso, C., Deruère, J, Kieber, JJ, and DeLong, A. 2006. RCN1-regulated phosphatase activity and EIN2 modulate hypocotyl gravitropism by a mechanism that does not require ethylene signaling. Plant Physiology: 141: 1617-1629
Buer CS, Sukumar P, and Muday GK. 2006. Ethylene induced flavonoid synthesis modulates root gravitropism. Plant Physiology: 140: 1384-1396
Sibout R, Sukumar P, Hettiarachchi C, Holm M, Muday GK and Hardtke CS. 2006. Opposite root growth phenotypes of hy5 vs. hy5 hyh correlate with increased constitutive auxin signaling. Plos Genetics.
Buer CS and Muday GK. 2004. The transparent testa4 mutation prevents flavonoid synthesis and alters auxin transport and the response of Arabidopsis roots to gravity and light. Plant Cell, 16:1191-1205